Dr. Ginsberg is a Research Scientist in NKI's Center for Dementia Research (CDR) and a tenured Associate Professor in the Departments of Psychiatry, Physiology and Neuroscience, New York University Grossman School of Medicine. His research interests include understanding molecular and cellular underpinnings of selective vulnerability within specific sets of forebrain neurons, particularly as they pertain to neurodegenerative, neuropsychiatric, and neurodevelopmental disorders. Dr. Ginsberg employs animal and cellular models of neurodegeneration and neurodevelopment, as well as human postmortem brain tissues for functional genomic and proteomic based studies relevant to understanding the pathobiology of aging, mild cognitive impairment (MCI), and Alzheimer’s disease (AD). The underlying hypothesis of his research is that individual cell types are likely to have unique patterns of gene and protein expression under normative conditions that are altered in pathological states, which drives subsequent neurodegeneration.
Dr. Ginsberg is trained in neuroanatomy, neuropathology, neurochemistry, and molecular and cellular neurobiology, including single-cell RNA amplification. He has been studying gene and protein expression profiles in the forebrain including the hippocampal formation, basal forebrain, and neocortex for over 21 years. He has over 20 years of experience with RNA amplification and microarray technology, and over 14 years of experience in proteomic profiling in animal and cellular models and human postmortem brains.
Current methodologies in his lab include confocal laser scanning microscopy, dietary manipulations including calorie restriction (CR) and maternal choline supplementation (MCS), electron microscopy, immunocytochemistry, in situ hybridization, microarray analysis, microaspiration via laser capture microdissection (LCM), Nanostring nCounter, Northern blotting, proteomics, real-time qPCR, regional gene amplification, RNA-seq, single cell/population cell gene amplification, surgical manipulations including axotomy and intracerebral delivery paradigms, and Western blotting. Dr. Ginsberg's multidisciplinary investment in performing expression profiling studies at the single population and subregional level is relevant towards understanding the potential pathogenesis of selective neuronal vulnerability in AD and relevant AD models.
Inda MC, Joshi S, Wang T, Bolaender A, Gandu S, Koren Iii J, Che AY, Taldone T, Yan P, Sun W, Uddin M, Panchal P, Riolo M, Shah S, Barlas A, Xu K, Chan LYL, Gruzinova A, Kishinevsky S, Studer L, Fossati V, Noggle SA, White JR, de Stanchina E, Sequeira S, Anthoney KH, Steele JW, Manova-Todorova K, Patil S, Dunphy MP, Pillarsetty N, Pereira AC, Erdjument-Bromage H, Neubert TA, Rodina A, Ginsberg SD, De Marco Garcia N, Luo W, Chiosis G. The epichaperome is a mediator of toxic hippocampal stress and leads to protein connectivity-based dysfunction. Nat Commun. 2020 Jan 16;11(1):319. doi: 10.1038/s41467-019-14082-5. PubMed PMID: 31949159; PubMed Central PMCID: PMC6965647.
Mufson EJ, Counts SE, Ginsberg SD, Mahady L, Perez SE, Massa SM, Longo FM, Ikonomovic MD. Nerve Growth Factor Pathobiology During the Progression of Alzheimer's Disease. Front Neurosci. 2019 Jul 1;13:533. doi: 10.3389/fnins.2019.00533. eCollection 2019. Review. PubMed PMID: 31312116; PubMed Central PMCID: PMC6613497.
Kelley CM, Ginsberg SD, Alldred MJ, Strupp BJ, Mufson EJ. Maternal Choline Supplementation Alters Basal Forebrain Cholinergic Neuron Gene Expression in the Ts65Dn Mouse Model of Down Syndrome. Dev Neurobiol. 2019 Jul;79(7):664-683. doi: 10.1002/dneu.22700. Epub 2019 Jun 9. PubMed PMID: 31120189; PubMed Central PMCID: PMC6756931.
Cox LM, Schafer MJ, Sohn J, Vincentini J, Weiner HL, Ginsberg SD, Blaser MJ. Calorie restriction slows age-related microbiota changes in an Alzheimer's disease model in female mice. Sci Rep. 2019 Nov 29;9(1):17904. doi: 10.1038/s41598-019-54187-x. PubMed PMID: 31784610; PubMed Central PMCID: PMC6884494.
Ginsberg SD, Malek-Ahmadi MH, Alldred MJ, Chen Y, Chen K, Chao MV, Counts SE, Mufson EJ. Brain-derived neurotrophic factor (BDNF) and TrkB hippocampal gene expression are putative predictors of neuritic plaque and neurofibrillary tangle pathology. Neurobiol Dis. 2019 Dec;132:104540. doi: 10.1016/j.nbd.2019.104540. Epub 2019 Jul 23. PubMed PMID: 31349032; PubMed Central PMCID: PMC6834890.